P1: Exploring the link between the microbiota and gut distal cancer
Principal Investigator Dirk Brenner
Host Institution LIH
Project Research Question: What is the link between the gut microbiota and lymphoma development?
Abstract
Peripheral T cell lymphoma are aggressive malignancies that represent ~15% of non-Hodgkin lymphomas and originate from a mature NKT or T cell. It is well established that the microbiome impacts the immune system and there is accumulating evidence of an existing link between the gut microbiota and lymphoma development. However, the nature of such a link is obscure. We aim to use a defined synthetic microbiota in combination with various genetic mouse models that develop peripheral T cell lymphoma to elucidate a distinct composition of intestinal bacteria that can limit lymphoma establishment. By various genetic and immunological approaches we further aim to identify a gut specific signal that mitigates lymphoma development and how this signal is transmitted.
Methods
Synthetic gut microbial communities, gnotobiotic and genetically altered mouse models, flow cytometry and immunological profiling methods, techniques in immunofluorescence and bioluminescence, histology, any large scale analyses (metabolomics, epigenetics, RNA-Seq., etc.) that can be applied in the scope of a defined question
